|
KMID : 0043320150380081506
|
|
Archives of Pharmacal Research
2015 Volume.38 No. 8 p.1506 ~ p.1511
|
|
|
Separation of two major chalcones from Angelica keiskei by high-speed counter-current chromatography
|
|
Kil Yun-Seo
Nam Joo-Won
Lee Jun
Seo Eun-Kyoung
|
|
|
Abstract
|
|
|
|
Angelica keiskei (Shin-sun cho) is an edible higher plant with the beneficial preventive effects on cancer, hypertension, and coronary heart disease. Two bioactive chalcones of Shin-sun cho, xanthoangelol (1) and 4-hydroxyderricin (2), were separated simultaneously by using high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane?EtOAc?MeOH?H2O (9:5:9:4). Only nonconsuming processes, solvent fractionations and Sephadex LH-20 column chromatography, were conducted as presteps. Xanthoangelol (1, 35.9 mg, 99.9 % purity at 254 and 365 nm) and 4-hydroxyderricin (2, 4.4 mg, 98.7 % purity at 254 nm and 98.8 % purity at 365 nm) were successfully purified from 70 mg of the processed extract from A. keiskei. The structures of two compounds were confirmed by 1H- and 13C-NMR analysis.
|
|
|
KEYWORD
|
|
|
HSCCC, Chalcone, Xanthoangelol, 4-Hydroxyderricin, Angelica keiskei, Umbelliferae
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|